Placeholder image of a protein
Icon representing a puzzle

529: CO2 and You!

Closed since over 10 years ago

Advanced Overall Design


March 14, 2012
Max points

Help fix this novel carbon-fixing enzyme. Try varying loop length and structures to make better scoring interactions with the ligand. In this puzzle, the first of a series, up to 7 residues can be added, and up to 10 can be removed. If you are new to Foldit, make sure you have completed Intro Puzzle 5-2, 7-1 through 7-4, 8-1 & 8-2 before trying this Advanced Science Puzzle. More info in the puzzle comments.

Top groups

  1. Avatar for Russian team 100 pts. 14,492
  2. Avatar for Contenders 2. Contenders 86 pts. 14,308
  3. Avatar for Anthropic Dreams 3. Anthropic Dreams 74 pts. 14,300
  4. Avatar for Void Crushers 4. Void Crushers 63 pts. 14,237
  5. Avatar for FoldIt@Netherlands 5. FoldIt@Netherlands 53 pts. 14,154
  6. Avatar for L'Alliance Francophone 6. L'Alliance Francophone 44 pts. 14,135
  7. Avatar for SETI.Germany 7. SETI.Germany 37 pts. 14,089
  8. Avatar for A970 8. A970 31 pts. 14,051
  9. Avatar for foldeRNA 9. foldeRNA 25 pts. 14,044
  10. Avatar for Go Science 10. Go Science 21 pts. 14,023

  1. Avatar for Grom
    1. Grom Lv 1
    100 pts. 14,478
  2. Avatar for meatexplosion 2. meatexplosion Lv 1 99 pts. 14,299
  3. Avatar for Mark- 3. Mark- Lv 1 98 pts. 14,248
  4. Avatar for gdnskye 4. gdnskye Lv 1 97 pts. 14,239
  5. Avatar for spdenne 5. spdenne Lv 1 96 pts. 14,237
  6. Avatar for mat747 6. mat747 Lv 1 95 pts. 14,224
  7. Avatar for Bletchley Park 7. Bletchley Park Lv 1 94 pts. 14,224
  8. Avatar for steveB 8. steveB Lv 1 93 pts. 14,196
  9. Avatar for tokens 9. tokens Lv 1 92 pts. 14,188
  10. Avatar for BitSpawn 10. BitSpawn Lv 1 91 pts. 14,178


brow42 Lv 1

Just a heads up, I think segments 33, 60, 61, 119, 169, 248, 380, and 410 are what rav3n calls 'wiggle locked'. Bands to these are ignored.

CEiben Lv 1

Designing new loops and interactions for ligand 420 (C1) is preferred over 419 (B1). It's not clear a priori what kinds of interactions will help so I recommend being creative.

I'm having trouble reproducing the 'wiggle lock'. When I wiggle after banding between 33 and 60, the residues move together on my computer. More information on how to reproduce this lock would be very helpful since it's not intentional in this puzzle.

brow42 Lv 1

I test these by making a strength 10 band between the segment and a locked segment, such as 1. I then do wiggle for 1 iteration, and check the change in score. Then I quickload for the next segment. The listed segments all show a change in score identical the unbanded case.

In the latest flu design puzzle, all the wiggle locked segments had constraints, but not all of the segments with constraints were locked. That was the first time I used this method.

I modified for the new puzzle, but didn't upload it because my main client is doing other stuff. I can probably upload a new version in the morning.

mottiger Lv 1

is a consecutive numbering of the editable segments possible?
as it is right now, it is impossible to run a rebuilding script or at least it is not possible to run my top fav: DRW.

mbinfield Lv 1

Question based on my total ignorance of dynamics at this scale: is there any reason to worry about how the ligands move into place? They seem to be in the center of the protein, so I don't see an obvious path in or out. Is it possible for us to change the backbone in such a way that the ligands are nicely bonded into place, but there's no way for them to actually reach that position or leave it?

beta_helix Staff Lv 1

so hopefully it'll be fixed in the new Flu Puzzle that will be posted next week.

Thanks for your patience with this one.

beta_helix Staff Lv 1

but sadly there is no way for us to do this, Rosetta needs the entire chain to be numbered consecutively.
You'll need to work on rebuilding the loops individually when using that script instead of all of them at the same time.

mbinfield Lv 1

It's possible to make a rebuilding script that works on specified non-consecutive portions of the protein. You just need to talk Rav into making the necessary modifications to DRW (assuming it's not already in the options somewhere; I haven't looked.)