Icon representing a puzzle

2199: Two-sided Strand Binder Design: Round 5

Closed since 16 days ago

Intermediate Overall Design

Summary


Created
September 14, 2022
Expires
Max points
100
Description

Design a beta strand and a separate protein that can bind to it! We've adjusted the H-bond Network Objective to encourage small but well-satisfied networks. We think the target strand needs just one buried, polar sidechain to achieve highly-specific binding. This puzzle allows 100 residues for the binder, and a Secondary Structure Objective will penalize designs with >50% helices. It will be important to design a binder that forms a strong beta sheet around the target strand.

This is a two-sided design problem, where we can design the sequence of both binding partners. The goal is to design a pair of proteins that will self-associate in solution. This is especially useful for bioengineering applications where we might want to use a short peptide as a "signal" that can be recognized by its binding partner. Although the short peptide may be flexible in solution, we would like to bind it in the shape of beta strand, so the target backbone has been frozen as a beta strand. However, you may still change the sidechains of the strand target to any amino acid you want!

AlphaFold predictions are enabled for the designed binder. Once you've designed a binder for the strand target, upload your solution for AlphaFold using the AlphaFold prediction tool. AlphaFold will predict the structure of your binder chain only (i.e. in the unbound state, in the absence of the strand target). If you load this prediction, then Foldit will attempt to align the prediction with your solution (i.e. in the bound state, making an interface with the strand target). If you continue working off of the AlphaFold prediction, you may need to make adjustments at the interface where the binder interacts with the target. See the puzzle comments for additional Objectives.

Top groups


  1. Avatar for Contenders 100 pts. 19,908
  2. Avatar for Anthropic Dreams 2. Anthropic Dreams 60 pts. 19,612
  3. Avatar for Go Science 3. Go Science 33 pts. 19,342
  4. Avatar for Gargleblasters 4. Gargleblasters 17 pts. 19,200
  5. Avatar for Marvin's bunch 5. Marvin's bunch 8 pts. 18,789
  6. Avatar for Australia 6. Australia 4 pts. 18,687
  7. Avatar for FamilyBarmettler 7. FamilyBarmettler 2 pts. 18,294
  8. Avatar for Ogre's lab 8. Ogre's lab 1 pt. 16,634
  9. Avatar for L'Alliance Francophone 9. L'Alliance Francophone 1 pt. 14,747
  10. Avatar for Foldit Staff 10. Foldit Staff 1 pt. 3,346

  1. Avatar for spvincent
    1. spvincent Lv 1
    100 pts. 19,882
  2. Avatar for gmn 2. gmn Lv 1 93 pts. 19,608
  3. Avatar for Galaxie 3. Galaxie Lv 1 86 pts. 19,519
  4. Avatar for dcrwheeler 4. dcrwheeler Lv 1 80 pts. 19,473
  5. Avatar for phi16 5. phi16 Lv 1 74 pts. 19,376
  6. Avatar for alcor29 6. alcor29 Lv 1 68 pts. 19,375
  7. Avatar for stomjoh 7. stomjoh Lv 1 63 pts. 19,365
  8. Avatar for zippyc137 8. zippyc137 Lv 1 58 pts. 19,228
  9. Avatar for infjamc 9. infjamc Lv 1 53 pts. 19,217
  10. Avatar for guineapig 10. guineapig Lv 1 49 pts. 19,206

Comments


bkoep Staff Lv 1

Objectives

DDG Metric (max +2000)
The predicted binding energy of your design. The goal DDG is -40.0 or less.

Contact Surface (max +2000)
Measures how much of your binder surface is in close contact with the target strand. The goal Contact Surface is 500 or more.

Buried Unsats (max +500)
Penalizes 20 points for each polar atom that cannot make any hydrogen bonds.

Core Existence (max +1500)
Ensures that at least 25 percent of all residues are buried in the core, when the binder is bound to the target.

H-bond Network (max +800)
Rewards networks that comprise at least 2 H-bonds involving core residues.
Between 1 and 2 H-bonds should cross the interface between target and binder.
Networks must be at least 75% satisfied (i.e. 75% of all bondable atoms in a network must make a H-bond).

Interaction Energy (max +500)
Monitors that all large PHE, TYR, and TRP residues are scoring well.

SS Design (max +500)
Disabled use of CYS residues.

Secondary Structure (max +500)
No more than 50% of residues may form helices. Extra helices are penalized at 10 points per residue.

Neural Net (max +0)
Achieve an AlphaFold prediction confidence of 80% or more.

stomjoh Lv 1

Posted scoring issues? My current high score is 19,087 since last night when I logged off. Top Soloists list shows my score at 11,586

HuubR Lv 1

The Description says "This puzzle allows 100 residues for the binder", but it seems that this 100 is the total number, including the 10 residues of the target. So we have "only" 90 residues for our binder, which is 10 less than Round 4 (Puzzle 2190), and 10 more than Rounds 1, 2, and 3.

We can use Delete and Insert, but we cannot insert beyond the initial number of 10 + 90 residues.