Placeholder image of a protein
Icon representing a puzzle

2047: Symmetric Tetramer Design with AlphaFold Predictions

Closed since about 1 year ago

Intermediate Overall Design Symmetry


September 22, 2021
Max points

Design a symmetric protein tetramer, with 4 identical chains of 90 residues each! This puzzle enables AlphaFold predictions for the monomer subunit of your design, so you can upload your solution for AlphaFold using the AlphaFold prediction tool. AlphaFold will predict the structure of your monomer subunit only (i.e. in the unbound state, in the absence of other symmetric copies). If you load this prediction, then Foldit will attempt to align the prediction with your solution. If you continue working off of the AlphaFold prediction, you may need to make adjustments at the interface where the monomer subunit interacts with symmetric copies.

This puzzle includes a Secondary Structure Objective, so no more than 50% of your design can form helices. The H-bond Network Objective encourages players to build buried, satisfied H-bond networks at the interface between symmetric chains. H-bond networks are a great way to introduce polar residues at the interface, but it's important that all of the bondable atoms make hydrogen bonds! We've also adjusted the H-bond Network Objective so that poor-scoring H-bonds may not contribute to networks; poor-scoring H-bonds will be displayed in red. This puzzle uses the Buried Unsats Objective, with a large penalty for buried polar atoms that can't make H-bonds. In this puzzle, there are no limits on the Complex Core, but we've included the Complex Core objective so players can see the core residues that can be incorporated into H-bond Networks.

Top groups

  1. Avatar for Contenders 100 pts. 24,868
  2. Avatar for Go Science 2. Go Science 70 pts. 24,663
  3. Avatar for Beta Folders 3. Beta Folders 47 pts. 24,035
  4. Avatar for Anthropic Dreams 4. Anthropic Dreams 30 pts. 23,927
  5. Avatar for Gargleblasters 5. Gargleblasters 19 pts. 23,166
  6. Avatar for Marvin's bunch 6. Marvin's bunch 11 pts. 22,447
  7. Avatar for Australia 7. Australia 7 pts. 19,005
  8. Avatar for L'Alliance Francophone 8. L'Alliance Francophone 4 pts. 18,130
  9. Avatar for Ogre's lab 9. Ogre's lab 2 pts. 16,648
  10. Avatar for BOINC@Poland 10. BOINC@Poland 1 pt. 16,446

  1. Avatar for ichwilldiesennamen 100 pts. 24,424
  2. Avatar for spvincent 2. spvincent Lv 1 95 pts. 24,269
  3. Avatar for dcrwheeler 3. dcrwheeler Lv 1 90 pts. 24,062
  4. Avatar for LociOiling 4. LociOiling Lv 1 86 pts. 24,035
  5. Avatar for Galaxie 5. Galaxie Lv 1 81 pts. 23,845
  6. Avatar for sgeldhof 6. sgeldhof Lv 1 77 pts. 23,842
  7. Avatar for Deleted player 7. Deleted player pts. 23,744
  8. Avatar for Bruno Kestemont 8. Bruno Kestemont Lv 1 68 pts. 23,459
  9. Avatar for BootsMcGraw 9. BootsMcGraw Lv 1 65 pts. 23,211
  10. Avatar for Enzyme 10. Enzyme Lv 1 61 pts. 23,166


bkoep Staff Lv 1

Buried Unsats (max +500)
Penalizes polar atoms that cannot make hydrogen bonds, -200 points per atom (not including symmetric copies).

Core Existence: Monomer (max +2000)
Ensures that at least 28 residues are buried in the core of the monomer unit.

Core: Complex (max +0)
Awards no bonuses or penalties. Click Show to see which residues count as "Core" for the H-bond Network objective.

H-bond Network (max +2400)
Rewards networks that comprise at least 2 H-bonds involving core residues.
Between 1 and 12 H-bonds should cross the interface between symmetric units.
Networks must be at least 75% satisfied (i.e. 75% of all bondable atoms in a network must make a H-bond).

Interaction Energy (max +500)
Monitors that all large PHE, TYR, and TRP residues are scoring well.

SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA in helices.

Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.

Secondary Structure (max +500)
No more than 50% of residues may form helices. Extra helices are penalized at 10 points per residue.