Questions about Chicken Anemia Virus (puzzle 731b)
|Opened on:||Friday, June 21, 2013 - 19:20|
|Last modified:||Saturday, June 22, 2013 - 16:38|
The only pictures I can find (by a layman's search of the web) of CRM1-binding NES (as mentioned in the comment on the blog post) seem to be of helices. Are there other examples of proteins with sheets that perform this function? (Would love an answer to this even if it's after the puzzle has closed.)
The Zhang predictions we were given have the isoleucines in residues 30-46 (which I gather are the active part of the NES) pretty deeply buried. Is it possible (maybe with Rosetta?) to generate predictions that have them nearer the surface (only slightly buried)? I have been trying to position them near the surface, with just a single "flap" over them that might pull away during multimerization, but doing that changes the protein pretty radically from the zhang predictions, to the point where any predictive value in the remainder of the zhang prediction is probably lost and I may as well fold it completely de novo.