puzzle picture
542: Advanced Alignment Cutpoint Puzzle
Status: Closed


276guineapig 16 14  7,8311
277kiman9292 46 1295  7,8281
278marie_s 46 1295  7,8071
279Megaviv 46 1295  7,7991
280nelsonlu 46 1295  7,7951
281koryu0609 46 1295  7,7701
282Willman1107 46 1295  7,7661
283Hebrew Hitman 46 373  7,7641
284hayange 46 1295  7,7601
285UnderJoyed 46 1295  7,7581
286wyospartan117 46 1295  7,7511
287Basse 46 1295  7,7481
288alex_book 46 1295 Ukraine7,7481
289GigiSlo 46 1295  7,7421
290anderscridland 46 1295  7,7411
291Alex333 46 572  7,7401
292donutvampire 46 1295  7,7381
293misterejay 46 1295  7,7361
294dgolow 46 1295  7,7361
295versat82 46 706 Piratenpartei Deutschland7,7351
296everlast2504 46 1295  7,7341
297birdy_ssr 46 1295  7,7341
298isopod3 46 1295  7,7341
299bobfin 46 1295  7,7341
300crawlee34225 46 1295  7,7341

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beta_helix's picture
User offline. Last seen 2 hours 5 min ago. Offline
Joined: 05/09/2008
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Good practice for CASP 10

This puzzle is actually a CASP 9 target (T0552), which some of you might recognize from previous Foldit puzzles. (The results from previous puzzles finally led us to implementing the new cutpoints).

The native has been released, but it was solved using NMR and the ensemble of models has a lot of variation (as you can see below, this is WITH the termini trimmed):

NMR ensemble of 2L3B: First 10 models in NMR ensemble for 2L3BNMR ensemble of 2L3B: First 10 models in NMR ensemble for 2L3B

You can obviously examine the native all you want (and for fairness we are posting it here in the puzzle comments: http://www.rcsb.org/pdb/explore/explore.do?structureId=2l3b) but the goal of this puzzle is to help us all prepare for CASP 10, which starts in early May!

Clearly for CASP, there will be no natives, so we want to find out now if the current Alignment Tool is sufficient to perform well on the Template-Based targets.

WE KNOW THAT YOU WANT A SCISSOR TOOL TO PUT IN YOUR OWN CUTPOINTS :-) That will be added soon, but we want to make sure that the current cutpoint system does not have any major bugs.

Although you can just thread the starting alignment and massage/fold the protein to a decent conformation, we believe that initially moving the alignment around can result in a better starting model (making it easier to get close to the native). Obviously, this is what we want to find out... so please let us know!

Thank you and happy threading!

spmm's picture
User offline. Last seen 2 weeks 1 day ago. Offline
Joined: 08/05/2010
Groups: Void Crushers
puzzle 369

would seem to be the earlier puzzle referred to in the text above. High scores there were 11062.xxx

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