Ligand fans will be disappointed to learn that terbium was unable to appear in this puzzle due to a scheduling conflict.
Instead, there's a terbium-size hole in there somewhere.
Terbium will be green-screened in during post-production.
In this puzzle, the Insert and Delete Segment actions are enabled, depending on the selected segment(s).
Tried to use these actions:
- Insert "cannot insert any more" (unless I tell it to insert zero segments, but that is not very useful). That makes sense: I cannot use more than the given 65 segments.
- So I have to use Delete first? The first and last segments are not deletable, nor are the segments near the binding site, and when I select any other segment (one or more), Delete causes an immediate crash.
As a result, this puzzle is restricted to having the binding site in the exact centre of a 65 residue chain. I would say that is an unnecessary and unwanted restriction, but maybe someone can explain the reason?
Thanks for the feedback! I'm able to reproduce the crash here, but it's unlikely we'll have a fix ready before the puzzle expires.
Practically speaking, this unfortunately means that the binding site is fixed in the center of the chain for this puzzle. We'll try to get this fixed so we can do another round in the future!
Buried Unsats (max +500)
Penalizes polar atoms that cannot make hydrogen bonds, -150 points per atom (not including symmetric copies).
Core Existence: Monomer (max +1700)
Ensures that at least 17 residues are buried in the core of the monomer unit.
Core: Complex (max +0)
Awards no bonuses or penalties.
Interaction Energy (max +500)
Monitors that all large PHE, TYR, and TRP residues are scoring well.
SS Design (max +500)
Penalizes all CYS residues.
Neural Net (max +0)
Achieve an AlphaFold prediction confidence of 80% or more.