2133: Symmetric Trimer Design with AlphaFold Predictions
|Name:||2133: Symmetric Trimer Design with AlphaFold Predictions|
|Expired:||04/20/2022 - 23:00|
|Description:||Note: This puzzle will crash if a player uses the Cut tool. The Foldit team has tracked down the bug and will release a software update shortly. In the meantime, please avoid using the Cut tool on this puzzle!
Design a symmetric protein trimer, with 3 identical chains that assemble together! This puzzle enables AlphaFold predictions for the monomer subunit of your design, so you can upload your solution for AlphaFold using the AlphaFold prediction tool. AlphaFold will predict the structure of your monomer subunit only (i.e. in the unbound state, in the absence of other symmetric copies). If you load this prediction, then Foldit will attempt to align the prediction with your solution. If you continue working off of the AlphaFold prediction, you may need to make adjustments at the interface where the monomer subunit interacts with symmetric copies.
This puzzle includes a Secondary Structure Objective, so no more than 50% of your design can form helices. The H-bond Network Objective encourages players to build buried, satisfied H-bond networks at the interface between symmetric chains. H-bond networks are a great way to introduce polar residues at the interface, but it's important that all of the bondable atoms make hydrogen bonds! We've also adjusted the H-bond Network Objective so that poor-scoring H-bonds may not contribute to networks; poor-scoring H-bonds will be displayed in red. This puzzle uses the Buried Unsats Objective, with a large penalty for buried polar atoms that can't make H-bonds. In this puzzle, there are no limits on the Complex Core, but we've included the Complex Core objective so players can see the core residues that can be incorporated into H-bond Networks.
|Categories:||Design, Overall, Symmetry|
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