1 reply [Last post]
Vincera's picture
User offline. Last seen 5 hours 7 min ago. Offline
Joined: 02/14/2010
Groups: Go Science

""...which is incredibly complex and requires many computations to calculate all possible angles for each peptide to fold, while taking into account electrostatic interactions and solvent effects.

As an example of the effect solvents have, hydrophobic pockets in proteins are often tucked inside the protein— far away from any water.

A semi-practical example to show what I mean in real-life terms is that a protein, with a three-dimensional structure resulting from the folding of its individual amino acid constituents, once denatured (boiled to at least 95C), can then be approximated as being permanently useless— unable to serve its previous biochemical function.

This is the theory behind boiling water as an anti-microbial method— you’re able to denature any viral, or other, proteins which may be in solution, and the probability of them spontaneously refolding into their biochemically active state can be approximated to zero.

There are too many possible ways for the protein to fold, so spontaneous refolding is unlikely (in the statistical, computer-science sense) to reoccur in vitro.""

https://www.quora.com/What-are-the-mathematics-of-protein-folding

jeff101's picture
User offline. Last seen 1 day 6 hours ago. Offline
Joined: 04/20/2012
Groups: Go Science
Protein Denaturation & Renaturation

I think there is more to the anti-microbial effects of boiling water
than discussed above. Articles like below make it seem that protein 
denaturation can be reversed under appropriate conditions. In fact,
many biochemistry lab techniques depend on being able to renature 
proteins (get them to refold into their native conformations).

https://www.quora.com/What-is-meant-by-renaturation-of-protein-What-are-the-various-approach-towards-renaturation
https://www.biologyonline.com/dictionary/renaturation

Also, there are various diseases that are spread by eating special 
proteins, like prion proteins. 

https://www.cdc.gov/prions/index.html
https://www.hopkinsmedicine.org/health/conditions-and-diseases/prion-diseases
https://www.fda.gov/animal-veterinary/animal-health-literacy/all-about-bse-mad-cow-disease

Sitemap

Developed by: UW Center for Game Science, UW Institute for Protein Design, Northeastern University, Vanderbilt University Meiler Lab, UC Davis
Supported by: DARPA, NSF, NIH, HHMI, Amazon, Microsoft, Adobe, RosettaCommons