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1977: Symmetric Tetramer Design: H-bond Networks
Status: Closed

Summary

Name: 1977: Symmetric Tetramer Design: H-bond Networks
Status: Closed
Created: 04/07/2021
Points: 100
Expired: 04/14/2021 - 23:00
Difficulty: Intermediate
Description: Design a symmetric protein tetramer, with 4 identical chains that assemble together! This puzzle includes a Secondary Structure Objective, so no more than 50% of your design can form helices. The H-bond Network Objective encourages players to build buried, satisfied H-bond networks at the interface between symmetric chains. H-bond networks are a great way to introduce polar residues at the interface, but it's important that all of the bondable atoms make hydrogen bonds! We've also adjusted the H-bond Network Objective so that poor-scoring H-bonds may not contribute to networks; poor-scoring H-bonds will be displayed in red. This puzzle uses the Buried Unsats Objective, with a large penalty for buried polar atoms that can't make H-bonds. In this puzzle, there are no limits on the Complex Core, but we've included the Complex Core objective so players can see the core residues that can be incorporated into H-bond Networks.
Categories: Design, Overall, Symmetry

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1Go Science23,052100
2Contenders22,70776
3Anthropic Dreams21,74156
4Void Crushers21,41141
5Beta Folders21,04029

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Comments

bkoep's picture
User offline. Last seen 11 hours 12 min ago. Offline
Joined: 11/15/2012
Groups: Foldit Staff
Objectives

Buried Unsats (max +500)
Penalizes polar atoms that cannot make hydrogen bonds, -200 points per atom (not including symmetric copies).

Core Existence: Monomer (max +2200)
Ensures that at least 22 residues are buried in the core of the monomer unit.

Core: Complex (max +0)
Awards no bonuses or penalties. Click Show to see which residues count as "Core" for the H-bond Network objective.

H-bond Network (max +2400)
Rewards networks that comprise at least 2 H-bonds involving core residues.
Between 1 and 12 H-bonds should cross the interface between symmetric units.
Networks must be at least 75% satisfied (i.e. 75% of all bondable atoms in a network must make a H-bond).

Interaction Energy (max +500)
Monitors that all large PHE, TYR, and TRP residues are scoring well.

SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA in helices.

Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.

Secondary Structure (max +500)
No more than 50% of residues may form helices. Extra helices are penalized at 10 points per residue.

Joined: 06/06/2013
Groups: Gargleblasters
puzzle not listing in main version

did puzzle get lost? can we send Trigger to kick start the server? Going to watch a movie instead

Joined: 12/06/2008
Groups: Contenders
Trigger confined to home.

Trigger and I are confined to home, as we are showing signs of COVID, and are awaiting test results.

LociOiling's picture
User offline. Last seen 22 min 21 sec ago. Offline
Joined: 12/27/2012
Groups: Beta Folders
puzzle ferrets in wildcat strike

Break out the peanut butter. Meanwhile, it's just me and MVY in devprev. MVY is winning.

LociOiling's picture
User offline. Last seen 22 min 21 sec ago. Offline
Joined: 12/27/2012
Groups: Beta Folders
oops fixed now

jflat06 fixed it, moments before I got around to complaining

beta_helix's picture
User offline. Last seen 11 hours 25 min ago. Offline
Joined: 05/09/2008
Groups: None
the system works!

;-)

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