puzzle picture
1907: MERS-CoV Binder Design
Status: Closed

Summary

Name: 1907: MERS-CoV Binder Design
Status: Closed
Created: 10/22/2020
Points: 100
Expired: 10/29/2020 - 23:00
Difficulty: Intermediate
Description: Design a binder for the viral spike of MERS-CoV! MERS is a respiratory disease similar to COVID-19, and is caused by a related coronavirus. There have been several MERS outbreaks since 2012, but there is still no treatment has been developed for the disease. The MERS-CoV virus is coated with a spike protein that recognizes the human protein DPP4, which is found on the surface of lung cells. A protein that binds to the MERS-CoV spike could be used as a drug to block DPP4 recognition and slow viral infection.

In this puzzle, players are presented with the binding site of the MERS-CoV spike protein. The backbone and most of the sidechains are completely frozen, except for flexible sidechains at the binding site, where the spike protein normally interacts with the human receptor protein. Players can design a new protein that binds to these sidechains, blocking interactions with the human receptor. In order to bind the MERS-CoV target, designs will need to make lots of hydrophobic contacts and satisfy any polar atoms that are buried at the interface. But designs will also need to have lots of secondary structure (helices or sheets) and a large core, so that they fold up correctly! Binder DDG, SASA, and SC are available as optional Objectives, but will award no bonus; a follow-up puzzle will include these as Foldit Metrics. See the puzzle comments for Objective details.
Categories: Design, Overall

Top Groups

RankGroupScorePoints
1Gargleblasters14,229100
2Go Science14,08673
3Anthropic Dreams13,98852
4Contenders13,96736
5Beta Folders13,95424

Top Evolvers

Top Soloists

RankPlayerGroupScorePoints
1Enzyme 74 Gargleblasters14,204100
2ichwilldiesennamen 5 Go Science14,08697
3dcrwheeler 98  14,08494
4johnmitch 98  14,06291
5Mike Lewis 10 Gargleblasters14,03088


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Comments

bkoep's picture
User offline. Last seen 3 hours 55 min ago. Offline
Joined: 11/15/2012
Groups: Foldit Staff
Objectives

DDG Score (max +0)
The predicted binding energy of your design. The target DDG is -40.0 or less.

SASA Score (max +0)
The solvent-accessible surface area that is lost upon binding. The target SASA is 1500 or more.

Shape Complementarity (max +0)
Measures how well your binder fits the contours of the target surface. The target Shape Complementarity is 0.60 or more.

Buried Unsats (max +500)
Penalizes 60 points for each polar atom that cannot make any hydrogen bonds.

Residue Count (max +275)
Penalizes extra residues inserted beyond the starting 161, at a cost of 55 points per residue. Players may use up to 166 residues in total.

Core Existence (max +1500)
Ensures that at least 25 percent of residues are buried in the core of the monomer unit.

Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.

SS Design (max +500)
Disabled use of CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA in helices.

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Developed by: UW Center for Game Science, UW Institute for Protein Design, Northeastern University, Vanderbilt University Meiler Lab, UC Davis
Supported by: DARPA, NSF, NIH, HHMI, Amazon, Microsoft, Adobe, RosettaCommons