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1903: Two-sided Interface Design
Status: Closed
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Summary
| Name: |
1903: Two-sided Interface Design |
| Status: |
Closed |
| Created: |
10/14/2020 |
| Points: |
0 |
| Expired: |
10/21/2020 - 23:00 |
| Difficulty: |
Intermediate |
| Description: |
Note: This puzzle was closed early due to problems in the puzzle setup. A corrected version has been posted as Puzzle 1903b.
Design an interface between two protein chains! This puzzle is a little different from our regular design puzzles. The starting structure includes two helical hairpins that are frozen in place and separated from one another. We want to build on these hairpins to create a binding interface between the two protein chains. However, the starting position of the hairpins is important, so players cannot move the hairpins closer to one another. The only way to make the two chains bind will be to fold up a brand new interface between them.
As you build on the two hairpins, it will be important to make a strong core with each chain, so that the chains fold up correctly. It will also be important to have a strong core at the interface where the two chains come together, so that they bind each other tightly. Keep an eye on the Core Existence Objective, which tracks all of these core residues together. Players may add up to 20 additional residues at a cost of 55 points per residue. See the puzzle comments for Objective details. |
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Comments
Residue Count (max +1100)
Penalizes added residues at a cost of 55 pts per residue. Players may add up to 20 residues, for a total of 200.
Buried Unsats (max +500)
Penalizes polar atoms that cannot make hydrogen bonds, -150 points per atom (not including symmetric copies).
Core Existence: Total (max +5000)
Ensures that at least 25% of residues are buried in the core of the entire solution.
Interaction Energy (max +500)
Monitors that all large PHE, TYR, and TRP residues are scoring well.
SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, SER, THR in helices.
Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.