Buried Unsats (max +500)
Penalizes polar atoms that cannot make hydrogen bonds, -150 points per atom (not including symmetric copies).
Core Existence: Monomer (max +2200)
Ensures that at least 22 residues are buried in the core of the monomer unit.
Core Limit: Complex (max +500)
Checks that no more than 87 residues are buried in the symmetric complex, including the interface between monomer units.
SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA, SER, THR in helices.
Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.