Old loop Helix detected as non ideal loop and can be changed as Glycine

Case number:699969-2009976
Topic:General
Opened by:nspc
Status:Closed
Type:Suggestion
Opened on:Friday, July 10, 2020 - 22:17
Last modified:Sunday, September 6, 2020 - 00:39

After changed a loop into an Helix, I can change AA as Glycine on it, and it can be detected as an non ideal loop.

http://pcmappeur2.free.fr/foldit/forum/non_ideal_loop_helix.jpg

http://pcmappeur2.free.fr/foldit/forum/can_select_glycine.jpg

(Fri, 07/10/2020 - 22:17  |  4 comments)


bkoep's picture
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Joined: 11/15/2012
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To clarify, the Ideal Loops and SS Design Objectives detect helices based on H-bonding patterns. These Objectives will only treat your residue as a helix if the backbone makes an H-bond to another residue 3-4 residues away. You can use Auto Structures to see where these Objectives actually see a helix.

The "Assign Helix" tool is different, and it doesn't actually create the H-bonds that make a real helix. Assign Helix is a way for you to mark where a helix should be. Then you can use the Idealize SS tool to actually reshape the region that is Assigned Helix, so that those residues make the correct H-bonds.

I know this can be confusing. When Foldit was first released 12 years ago, it was designed to work with helices a bit differently than it is now. Foldit has changed significantly since then, and the Assign Helix feature is sort of a relic from the past, but still useful in some cases.

nspc's picture
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Joined: 03/26/2020
Groups: Go Science

Thanks. I understand more how its works now.

I thought secondary structure was in ADN code, but not at all. So, it become and Helix a Sheet or a Loop only with amino acids we choose, if I understand. It is the same atoms, just differents positions and bound ok. (I thought backbone was different).

That means, if a tripple helix is not enough stable, some residues can become a Loop or a Sheet instead of a Helix we wanted, in reality?

bkoep's picture
User offline. Last seen 23 hours 33 min ago. Offline
Joined: 11/15/2012
Groups: Foldit Staff

That's exactly right. The atoms (and chemical structure) of a helix and sheet are exactly the same. They are just folded up differently.

If you want your designed protein to fold into a 3-helix bundle, then you have to pick amino acids carefully so that the helix bundle is more stable than sheets or loops. If the amino acids are more stable in a sheet than in a helix, that means the amino acids will fold up into a sheet in reality.

This blog post goes into some deeper discussion about the theory of protein design.

Joined: 09/29/2016
Groups: Gargleblasters
Status: Open » Closed

Marking this closed.

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