Residue Count (max +550)
Penalizes extra residues inserted beyond the starting 194, at a cost of 55 points per residue. Players may use up to 204 residues in total.
Core Existence (max +2400)
Ensures that at least 28 percent of residues are buried in the core of the monomer unit.
Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.
SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA, SER, THR in helices.
When checking on the end locked loops from the given binding helix, I don't find ABEGO (Rama) realistic loops for segments 146 and 168.
Is that intended?
The very very low probability I find is Threonine for the 169 and glycine for the 146, both almost red (helix).
I've the impression that it will be difficult if not impossible to find an ideal loop on both sides of this helix (I found one on one side, but it crashed the SS backbones).