puzzle picture
1761: Symmetric Trimer Design: H-Bond Networks
Status: Closed

Summary

Name: 1761: Symmetric Trimer Design: H-Bond Networks
Status: Closed
Created: 11/18/2019
Points: 100
Expired: 11/25/2019 - 23:00
Difficulty: Intermediate
Description: H-bond Networks are back! The H-bond Network Objective encourages players to bury satisfied H-bond networks at the interface between symmetric chains. H-bond networks are a great way to introduce polar residues at the interface, but it's important that all of the bondable atoms make hydrogen bonds! In this puzzle, there are no limits on the Complex Core, but we've included the Complex Core objective so players can see which residues count as core in the H-bond Networks. There a few other objectives in effect; see the puzzle comments for details. The Baker Lab will run folding predictions on your solutions for this puzzle, and those that perform well will be synthesized in the lab. Remember, you can use the Upload for Scientists button for up to 5 designs that you want us to look at, even if they are not the best-scoring solutions!
Categories: Design, Overall, Symmetry

Top Groups

RankGroupScorePoints
1Contenders24,228100
2Go Science23,93470
3Gargleblasters23,92447
4Anthropic Dreams23,76430
5Void Crushers23,55719

Top Evolvers

Top Soloists



Need this puzzle? Log in to download.  

Comments

bkoep's picture
User offline. Last seen 1 hour 18 min ago. Offline
Joined: 11/15/2012
Groups: None
Objectives

Core Existence: Monomer (max +3000)
Ensures that at least 30 residues are buried in the core of the monomer unit.

Core: Complex (max +0)
Awards no bonuses or penalties. Click Show to see which residues count as "Core" for the H-bond Network objective.

H-bond Network: Rewards networks that comprise at least two H-bonds involving core residues. Networks must be at least 75% satisfied (i.e. 75% of all bondable atoms in a network must make a H-bond).

SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA in helices.

Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.

Joined: 12/07/2007
Groups: Contenders
H-bond Network bug?

I'm seeing what might be a scoring issue with H-bond networks: of 3 identical networks 2 score 500 and one scores 333.

Scientist-shared as "Possible HNet scoring bug"

bkoep's picture
User offline. Last seen 1 hour 18 min ago. Offline
Joined: 11/15/2012
Groups: None
Thanks for sharing!

That sure sounds like a bug—we'll take a look!

bkoep's picture
User offline. Last seen 1 hour 18 min ago. Offline
Joined: 11/15/2012
Groups: None
Maximum h-bonds

This is a quirk in the way that HBNetworks are scored, and something we can adjust in the future. This puzzle gives a bonus for maximum 8 H-bonds, but each of your networks has 3 H-bonds. Because of the 8 bond limit, one of your networks only gets a bonus from 2 of its H-bonds.

The HBNet bonus scales with the number of H-bonds in your network. This means that you can get a bigger bonus by adding H-bonds to your network. But in reality, the size of the network is not as important as its satisfaction (i.e. do all of the polar atoms make H-bonds). We limit the maximum H-bonds to try to prevent excessively large networks.

I realize this info about maximum H-bonds isn't available for players anywhere. We'll make sure to include it on future puzzles!

Joined: 12/07/2007
Groups: Contenders
Another possible issue (or

Another possible issue (or maybe a different manifestation of the same one). Changing a residue not involved in the HNet reduces its score. Scientist shared as "HNet Issue 2"

bkoep's picture
User offline. Last seen 1 hour 18 min ago. Offline
Joined: 11/15/2012
Groups: None
H-bonds not recognized in network

It looks to me like a bug. That residue probably should be involved in the HBNet.

In this screenshot, the bottom ARG is clearly making a H-bond with a SER in the network, even though the ARG is not counted in the network. If you mutate the ARG, then the SER becomes unsatisfied, reducing the score of the HBNet.

I'm not sure why the ARG is excluded from the network. I can think of a couple places where something might be going wrong, but we'll have to dig a little deeper to figure it out.

Get Started: Download
  Windows    OSX    Linux  
Windows
(7/8/10)
OSX
(10.7 or later)
Linux
(64-bit)

Are you new to Foldit? Click here.

Are you a student? Click here.

Are you an educator? Click here.
Search
Only search fold.it
Recommend Foldit
User login
Soloists
Evolvers
Groups
Topics
Top New Users
Sitemap

Developed by: UW Center for Game Science, UW Institute for Protein Design, Northeastern University, Vanderbilt University Meiler Lab, UC Davis
Supported by: DARPA, NSF, NIH, HHMI, Amazon, Microsoft, Adobe, RosettaCommons