Core Existence: Monomer (max +2000)
Ensures that at least 20 residues are buried in the core of the monomer unit.
Core Existence: Complex (max +2400)
Ensures that at least 48 residues are buried in the core of the entire complex (including the interface between monomer units).
SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA, SER, THR in helices.
Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.