Core Existence: Monomer (max +2500)
Ensures that at least 25 residues are buried in the core of the monomer unit.
Core Existence: Complex (max +3100)
Ensures that at least 62 residues are buried in the core of the entire complex (including the interface between monomer units).
SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets and helices
Secondary Structure (max +500)
No more than 50% of residues may form helices. Extra helices are penalized at 10 points per residue.
Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.
just a note that some of my "ideal loops" showed up as non-ideal even with blueprint tool holding them locked.... I think it is me, but it is frustrating
Can you share those solutions with us? Or let us know which Building Blocks are setting off the Ideal Loops objective?