Residue Count: Normalizes for extra residues inserted beyond the starting 70, at a cost of 32 points per residue. Players may use up to 100 residues in total.
Secondary Structure: Only 10% of residues may form helices. Additional helices will incur a penalty of 10 points per residue.
In Puzzle 1566: Small Monomer Design,
we had the Objectives below:
Interaction Energy: Monitors that all
PHE, TYR, and TRP residues are scoring well.
SS Design: Prohibits all CYS residues.
Prohibits GLY, ALA residues in sheets.
Prohibits GLY, ALA, SER, THR in helices.
Does 1569 have any of these restrictions?
Do you want 1569 to allow disulfide bonds,
That's correct; there is no Interaction Energy or SS Design objective in this puzzle.
However, CYS residues have been disabled in this puzzle, so players should not be able to design disulfide bonds.
bit odd that players were not told this at the start - don't u think?
Mutate is putting in too many tyrosines, and in the absence of a filter, the script I am running is keeping them.
To make sure your solutions have sequences that can't easily be
rebuilt as helices, it may help to include tryptophan, tyrosine,
phenylalanine, isoleucine, valine, and threonine residues that
says prefer to adopt β-strand conformations. It may also help
to include glycine and proline (helix-breaker) residues and to
avoid the helix-favoring MALEK (methionine, alanine, leucine,
glutamate, and lysine) residues. Another idea is to use
sequences that alternate between hydrophilic and hydrophobic
residues, as often occurs in sheets, rather than other patterns
that often occur in helices.
can we submit more than one?
Each player may submit only one design for the tournament. If a player submits multiple solutions with the title "Tournament Submission", then only the most recent submission will be considered for Phase Two of the tournament. The reason for this is to make sure everyone has an equal chance in the Phase Two selections.
However, we still want to see your designs! If you develop several designs that you like, please share them all with us using the Upload for Scientist button! Even though we will only consider one submission for the tournament, we will still run our regular analysis on all designs from this puzzle. We always look at the scientist-shares in design puzzles, and it's especially helpful if you can save them with an informative title and description!
OK, I wondered because if you wiggle the preferred solution on Medium you may not be able to do much with it the second round.