Disulfide bonds are very hard to create at CI 1
|Opened by:||Timo van der Laan|
|Opened on:||Tuesday, March 20, 2018 - 21:09|
|Last modified:||Thursday, March 22, 2018 - 19:54|
The standard tool I used to do this was bridge wiggle. But when running at CI 1 with the CYS only 2.6 apart bridge wiggle no longer manages to close the bridge. Also connecting the tips with a band strength 10 did not managed to do that.
My two cents: maybe the new scoring function makes a too great class to overcome.
Also pulling by hand never made the bridge. The only way I found was using cuts and move.
Others have managed to make bridge but they where working on low CI.
Can the scoring function be changed when clashing between the tips of CYS is concerced?