puzzle picture
1155: gp120 Design: V3 Hairpin
Status: Closed

Summary

Name: 1155: gp120 Design: V3 Hairpin
Status: Closed
Created: 11/04/2015
Points: 100
Expired: 11/09/2015 - 23:00
Difficulty: Intermediate
Description: This protein is a portion of the HIV-1 envelope glycoprotein. The HIV-1 Env protein is a trimer of heterodimers--gp120 (the receptor binding domain) and gp41 (the transmembrane and fusion domain)--and is found on the surface of the HIV-1 virus. The HIV-1 Env complex is responsible for recognizing and binding to a human immune cell through its receptors. When gp120 binds to the human receptors (CD4 and CXCR4 or CXCR5), dramatic conformational changes occur in gp120 and gp41 that cause the virus to fuse with and infect the human cell. This structure of gp120, in a pre-fusion conformation, was recently obtained by x-ray crystallography. However, we know that the V3 loop of gp120 (which exists as a beta-hairpin here) can move in solution, allowing binding of antibodies that will clash with this pre-fusion conformation.

We're asking Foldit players to redesign the V3 hairpin to stabilize it in this pre-fusion conformation! Note that this protein exists as a trimer; stabilization of the hairpin may also be achieved through intermolecular contacts! See the puzzle comments for more details.
Categories: Design, Overall, Symmetry

Top Groups

RankGroupScorePoints
1Contenders10,713100
2Beta Folders10,69278
3L'Alliance Francophone10,67060
4Go Science10,66445
5Anthropic Dreams10,65233

Top Evolvers

Top Soloists



Need this puzzle? Log in to download.  

Comments

bkoep's picture
User offline. Last seen 7 hours 19 min ago. Offline
Joined: 11/15/2012
Groups: None
Constraints on the hairpin

This puzzle includes no extra filters, but we have enabled some constraints to keep the V3 hairpin close to its starting conformation. The rest of the gp120 protein has a fixed backbone.

Players may only mutate residues on the V3 hairpin.

jamiexq's picture
User offline. Last seen 8 hours 49 min ago. Offline
Joined: 07/31/2011
Cysteine bonds

Cysteines on hairpin will not bond with cysteines on backbone (frozen part)

stomjoh's picture
User offline. Last seen 9 weeks 3 days ago. Offline
Joined: 11/18/2012
Groups: None
Scoring Bug?

I asked on the forums, but I'll also ask here: If there is a scoring bug going on with this puzzle, shouldn't it be taken down/restarted with the bug fixed? Or, will the bug be fixed before the puzzle finishes?

Joined: 04/24/2014
Groups: None
Please see

http://fold.it/portal/node/2001423 Please see this post for updates. Thanks!

Get Started: Download
  Windows    OSX    Linux  
Windows
(7/8/10)
OSX
(10.7 or later)
Linux
(64-bit)

Are you new to Foldit? Click here.

Are you a student? Click here.

Are you an educator? Click here.
Search
Only search fold.it
Recommend Foldit
User login
Soloists
Evolvers
Groups
Topics
Top New Users
Sitemap

Developed by: UW Center for Game Science, UW Institute for Protein Design, Northeastern University, Vanderbilt University Meiler Lab, UC Davis
Supported by: DARPA, NSF, NIH, HHMI, Amazon, Microsoft, Adobe, RosettaCommons