Orange Guard lets you protect orange sidechains that foldit wants to turn blue, by freezing those sidechains before you start the script. It will try to mutate those sidechains, but only to other orange ones.

Orange Guard will *not* score better than regular DRemixW, since it limits the mutations that foldit is allowed to try. The more sidechains you protect by freezing them, the more the score is limited. So why run it? It prevents foldit from producing a protein with a long stretch of only blue sidechains (say, an entire edge sheet, or more than one turn of a helix). The scientists generally don't pick a protein for testing in Rosetta if it has an entire blue sheet or a long stretch of blue helix, because they know the protein will not fold up as designed.

Use Orange Guard if getting your protein tested is more important to you than getting the top score. Better still, run Orange Guard in one track and the regular script in another track (you can use the same script, just don't freeze any sidechains first). Then you can have the best of both worlds.

Alright so after looking at the code through my untrained eyes, I'm now uncertain if this is even a bug due to your Comment of "Used only during Initialization - after that use AllowedList instead."
This made me second guess everything, considering I lack a pre-Recipe save to compare this 16hrs-later result to. As originally for all I know, that Valine may have been a -phyllic. In which case, things have obeyed the code and there's no bug. That being said, I'm still leaving my original "report" below jsut in case.

On that note, I DIO have a request, if possible and if it wouldn't cause an epic slowdown in the recipe:
If an AA is changed to -phobic and there is a Gain, then re-run the Initialization so that the newly-set -phobic can also be protected!
Ideally a tickbox option so that one can use either-or method. :)

Either way, thanks again for these OG recipe variants!






[Original Message]
Just happened to stop its run after the last Fuze was close to my Best, as I was curious to see what the exact differences were...

The biggest was the 2 mutations, and one of them was a Threonine which originally had been a Valine... So it didn't properly Guard that Orange. :(

I can't say for certain if any other -phobics were mutated into -phyllics, but hopefully not.

My guess was an error in your tables; however, as I look at the code it SEEMS like that's all correct. (from my not-a-coder perspective)

The recipe also unfreezes the backbone when unfreezing the AA, instead of only the AA.

I had wanted to keep the structure of certain parts, to prevent remix and wiggling, but allow its AA to still be mutated to something still-Orange. Alas, now half of the backbones are also unfrozen :P

are you letting the recipe run in loop mode? I often let this default setting run. I haven't tried this yet... but double check. Otherwise, need to freeze SS and sidechain both to keep everything