Help the Worcester Polytechnic Institute with this unsolved protein!

Our lab has been contacted by scientists at the Worcester Polytechnic Institute working on an unsolved viral protein. They are hoping you can help them solve the structure of this important protein, here is their message to you:

Apoptin is a viral protein from the Chicken Anemia Virus (CAV). It is of special interest because it selectively targets transform (Cancer) cells and causes apoptosis (programmed cell death). However we do not know why it selectively kills cancer cells, a function which is tied to its 3D structure. Solving this protein structure would greatly advance research into apoptin’s functionality and development as a method to treat cancer.

We are giving you 5 Zhang Server models to start with for Puzzle 731b: Unsolved Chicken Anemia Virus Protein

Here is a link to the Zhang Server webpage for more details:

Attached is an article discussing the function properties of Apoptin.

Journal_of_Virology.pdf1.21 MB
( Posted by  beta_helix 46 949  |  Sat, 06/15/2013 - 23:54  |  1 comment )
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Even more info if you are interested!

Here is some additional information provided by the Worcester Polytechnic Institute:

-There is a Functional Nuclear Export Sequence near N-Terminus (likely within residues 30-46) that binds to CRM1 (Exportin 1)

- Our Secondary/Tertiary Structure Predictions imply that there are two beta sheets in this area.

-We think that there is a multimerization sequence in the same area (We know that the protein multimerizes with itself).

- Our Secondary/Tertiary predictions also imply that the NES residues are normally buried. We hypothesize that multimerization may be required to expose the residues (specifically 33-37 and 40-44).

-There is a functional Bipartate Nuclear Localization Sequence near the C-Terminus (likely within residues 86-121) that probably binds to Importin Alpha.

-Our Secondary/Tertiary Structure Predictions strongly imply that there are two helices in this area; one between 95-105 and another between 115-121.

-There is a sequence in this area that binds to the first subunit of the Anaphase Promoting Complex (APC1).

Several secondary and tertiary prediction (Zhang) programs were used to obtain this data. These two links are to secondary structure prediction programs:

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