Preliminary Results of CO2 Fixation Enzyme Puzzle

We are very excited and impressed with the models everyone was able to make for the CO2 Fixation enzyme puzzle! While we do not know if there is a right answer we saw a lot of your answers converge on a single confirmation that suggests to us that there is an actual energy minima and you guys found it. In addition the molecular details of this Foldit predicted conformation really make sense with the chemistry occurring in the active site.

We are currently planning on taking the models that you generated and using them as a starting point for evolving a new an improved enzyme. Before your solutions it was very difficult to know which of the 10 amino acids were pointing towards the active site, making it impossible to try the combinatorial set of mutations using standard evolutionary techniques with this protein (2e11 possibilities if we were doing it completely at random). Using these models we can make directed mutations and narrow it down to roughly 300 proteins which we can test. We will post more on the results that came from your modeling efforts as we generate them.

Thanks and keep up the great folding!

( Posted by  beta_helix 127 7154  |  Wed, 03/23/2011 - 01:46  |  2 comments )
0
Joined: 12/07/2007
Groups: Contenders
Thanks for the update: I

Thanks for the update: I think we all appreciate hearing the results of our efforts. Look forward to hearing more about how work on this enzyme develops.

Joined: 05/14/2010
Groups: None
Please!!! For god's sake,

Please!!! For god's sake, post a PDB file already of people's predictions

Download links:
  Windows    OSX    Linux  
Windows
(7/8/10)
OSX
(10.12 or later)
Linux
(64-bit)

Are you new to Foldit? Click here.

Are you a student? Click here.

Are you an educator? Click here.
Search
Only search fold.it
Other Games: Mozak
Recommend Foldit
User login
Topics
Top New Users
Sitemap

Developed by: UW Center for Game Science, UW Institute for Protein Design, Northeastern University, Vanderbilt University Meiler Lab, UC Davis
Supported by: DARPA, NSF, NIH, HHMI, Amazon, Microsoft, Adobe, RosettaCommons