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284: Mini-CASP 22 - Single Template
Status: Closed
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Soloists
| 1 | keypad5 174 13410 | Richard Dawkins Foundation | 11,500 | 100 | | 2 | Mark- 80 59 | Contenders | 11,134 | 96 | | 3 | steveB 121 20 | Void Crushers | 11,124 | 91 | | 4 | aap 174 13410 | Richard Dawkins Foundation | 10,961 | 87 | | 5 | vakobo 174 13410 | Russian team | 10,910 | 83 | | 6 | HillObserver 174 13410 | Void Crushers | 10,872 | 79 | | 7 | Bletchley Park 6 3 | Contenders | 10,854 | 75 | | 8 | mimi 4 12 | Contenders | 10,834 | 72 | | 9 | vertex 142 148 | Richard Dawkins Foundation | 10,808 | 68 | | 10 | Steven Pletsch 162 116 | Another Hour Another Point | 10,789 | 65 | | 11 | Susanne 57 111 | SETI.Germany | 10,776 | 62 | | 12 | CharlieFortsCon... 31 68 | Contenders | 10,752 | 59 | | 13 | BootsMcGraw 15 15 | Contenders | 10,704 | 56 | | 14 | aendgraend 60 216 | SETI.Germany | 10,699 | 53 | | 15 | mat747 69 34 | Void Crushers | 10,658 | 50 | | 16 | tallguy-13088 174 75 | | 10,501 | 48 | | 17 | Mr_Jolty 174 185 | Natural Abilities | 10,447 | 45 | | 18 | Dolichwier 76 559 | | 10,402 | 43 | | 19 | Brick 174 13410 | GreatScience | 10,378 | 41 | | 20 | weitzen 174 138 | | 10,377 | 38 | | 21 | jobo0502 174 117 | L'Alliance Francophone | 10,345 | 36 | | 22 | drjr 126 108 | | 10,341 | 34 | | 23 | TheGUmmer 58 33 | Void Crushers | 10,335 | 33 | | 24 | Madde 81 26 | Void Crushers | 10,335 | 31 | | 25 | calhoun 174 13410 | Crunching Family | 10,324 | 29 |
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We hope this puzzle will be useful for you to see how important it is to use the alignment tool to find the optimal alignment. Even with the same template, in this case 2fug (chain 5), you can see the very different starting alignments that are possible.
Unfortunately none of the alignments match the N-terminal end of the protein, and the secondary structure predictions (which also used 2fug and the similar structure of 3iam, chain 5 as well) don't help much with that region, but hopefully it will still be somewhat useful:
Here is the sequence logo predicted by the SAM server.

H = helix
E = sheet
C = loop (or coil)
The taller the letter at each position, the higher the probability of that specific secondary structure for that amino acid.
For example, the amino acid Phenylalanine at residue 53 is highly predicted to be part of a sheet. However, the Alanine at residue 40 is predicted to have an equal probability of being anything.
For reference, if anyone is interested in the templates:
http://www.rcsb.org/pdb/explore/explore.do?structureId=2FUG
http://www.rcsb.org/pdb/explore/explore.do?structureId=3IAM