155 open guide bug?
| Case number: | 845813-986295 |
| Topic: | Game: Tools |
| Opened by: | CharlieFortsCon... |
| Status: | Closed |
| Type: | Bug |
| Opened on: | Monday, June 15, 2009 - 23:02 |
| Last modified: | Saturday, January 7, 2012 - 00:17 |
Is there an error in the guides for 155 global open? There's the backbone guide, and the grey sidechain guide, and they dont seem to be agreeing at the res #1 end. Using the backbone guide to rebuild that res #1 end, then taking the sidechain grey shadows as an indicator, there seems to be a one residue difference in the placement of where the sidechains should be. 12-Val is easily placed, shadow matching the sidechain guide. But the next available hydrophobe, 16-Phe, seems to be one entire residue out in the side chain guide, which causes all kinds of ridiculous consequences. Am I making sense, or do I need a holiday??? CFC
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Sat, 01/07/2012 - 00:17
#2
| Status: Open » Closed |
closed. Implemented, resolved, no comments or votes since 2009
CFC:
First, I made the assumption that you meant Val #16 and Phe #12 rather than the other way around. While it's true that the guide for these two residues is almost an exact match in my structure with a score of 10632, I do admit that rebuilding the N-terminus (#1 end) of that puzzle took me quite a while on Day 1. In particular, it involved temporarily setting the 10-17 helix to loops and straightening the 5-6 sheet (in fact I set 4 and 7 to sheet as well) followed by rebuilding the 10-17 helix manually (i.e. freezing off the rest of the protein and using bands to set the location of each residue manually, followed by setting the band strength to 10 via a recipe and then wiggling).
==> You might lose a lot of points in the process, but once that N-terminus is corrected, the rest of the protein is easy...