Sat, 01/09/2021 - 02:16
#2
Strange filter message
Secondary structure -600
You have 110 too many residues in helices
This in a 4-helix bundle: whats happening?
Sat, 01/09/2021 - 04:52
#3
From the puzzle description:
From the puzzle description: "This puzzle includes a Secondary Structure Objective, so no more than 50% of your design can form helices."
Sat, 01/09/2021 - 16:05
#4
Tx spdenne: I should really
Tx spdenne: I should really try reading the puzzle description before posting!
Buried Unsats (max +500)
Penalizes polar atoms that cannot make hydrogen bonds, -200 points per atom (not including symmetric copies).
Core Existence: Monomer (max +2200)
Ensures that at least 22 residues are buried in the core of the monomer unit.
Core: Complex (max +0)
Awards no bonuses or penalties. Click Show to see which residues count as "Core" for the H-bond Network objective.
H-bond Network (max +2400)
Rewards networks that comprise at least 2 H-bonds involving core residues.
Between 1 and 12 H-bonds should cross the interface between symmetric units.
Networks must be at least 75% satisfied (i.e. 75% of all bondable atoms in a network must make a H-bond).
Interaction Energy (max +500)
Monitors that all large PHE, TYR, and TRP residues are scoring well.
SS Design (max +500)
Penalizes all CYS residues. Penalizes GLY, ALA residues in sheets. Penalizes GLY, ALA in helices.
Ideal Loops (max +500)
Penalizes any loop region that does not match one of the Building Blocks in the Blueprint tool. Use "Auto Structures" to see which regions of your protein count as loops.
Secondary Structure (max +500)
No more than 50% of residues may form helices. Extra helices are penalized at 10 points per residue.